Virus Isolation from the First Patient with SARS-CoV-2 in Korea.
Identifieur interne : 000165 ( Main/Exploration ); précédent : 000164; suivant : 000166Virus Isolation from the First Patient with SARS-CoV-2 in Korea.
Auteurs : Wan Beom Park [Corée du Sud] ; Nak Jung Kwon [Corée du Sud] ; Su Jin Choi [Corée du Sud] ; Chang Kyung Kang [Corée du Sud] ; Pyoeng Gyun Choe [Corée du Sud] ; Jin Yong Kim [Corée du Sud] ; Jiyoung Yun [Corée du Sud] ; Gir Won Lee [Corée du Sud] ; Moon Woo Seong [Corée du Sud] ; Nam Joong Kim [Corée du Sud] ; Jeong Sun Seo [Corée du Sud] ; Myoung Don Oh [Corée du Sud]Source :
- Journal of Korean medical science [ 1598-6357 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Infections à coronavirus, Pneumopathie virale.
- virologie : Partie orale du pharynx.
- Adulte, Femelle, Humains, Microscopie électronique à transmission, Phylogénie, République de Corée.
English descriptors
- KwdEn :
- MESH :
- geographic : Republic of Korea.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- genetics : Betacoronavirus.
- isolation & purification : Betacoronavirus.
- virology : Oropharynx.
- Adult, Female, Humans, Microscopy, Electron, Transmission, Phylogeny, Whole Genome Sequencing.
Abstract
Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.
DOI: 10.3346/jkms.2020.35.e84
PubMed: 32080990
Affiliations:
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University College of Medicine, Seoul, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Seo, Jeong Sun" sort="Seo, Jeong Sun" uniqKey="Seo J" first="Jeong Sun" last="Seo">Jeong Sun Seo</name><affiliation wicri:level="3"><nlm:affiliation>Macrogen Inc., Seoul, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Macrogen Inc., Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Oh, Myoung Don" sort="Oh, Myoung Don" uniqKey="Oh M" first="Myoung Don" last="Oh">Myoung Don Oh</name><affiliation wicri:level="3"><nlm:affiliation>Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Internal Medicine, Seoul National University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></analytic><series><title level="j">Journal of Korean medical science</title><idno type="eISSN">1598-6357</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Betacoronavirus (genetics)</term><term>Betacoronavirus (isolation & purification)</term><term>Coronavirus Infections (diagnosis)</term><term>Female</term><term>Humans</term><term>Microscopy, Electron, Transmission</term><term>Oropharynx (virology)</term><term>Phylogeny</term><term>Pneumonia, Viral (diagnosis)</term><term>Republic of Korea</term><term>Whole Genome Sequencing</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Femelle</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Microscopie électronique à transmission</term><term>Partie orale du pharynx (virologie)</term><term>Phylogénie</term><term>Pneumopathie virale (diagnostic)</term><term>République de Corée</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Republic of Korea</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term><term>Pneumopathie virale</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Betacoronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Betacoronavirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Partie orale du pharynx</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Oropharynx</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Female</term><term>Humans</term><term>Microscopy, Electron, Transmission</term><term>Phylogeny</term><term>Whole Genome Sequencing</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Femelle</term><term>Humains</term><term>Microscopie électronique à transmission</term><term>Phylogénie</term><term>République de Corée</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Park, Wan Beom" sort="Park, Wan Beom" uniqKey="Park W" first="Wan Beom" last="Park">Wan Beom Park</name></region><name sortKey="Choe, Pyoeng Gyun" sort="Choe, Pyoeng Gyun" uniqKey="Choe P" first="Pyoeng Gyun" last="Choe">Pyoeng Gyun Choe</name><name sortKey="Choi, Su Jin" sort="Choi, Su Jin" uniqKey="Choi S" first="Su Jin" last="Choi">Su Jin Choi</name><name sortKey="Kang, Chang Kyung" sort="Kang, Chang Kyung" uniqKey="Kang C" first="Chang Kyung" last="Kang">Chang Kyung Kang</name><name sortKey="Kim, Jin Yong" sort="Kim, Jin Yong" uniqKey="Kim J" first="Jin Yong" last="Kim">Jin Yong Kim</name><name sortKey="Kim, Nam Joong" sort="Kim, Nam Joong" uniqKey="Kim N" first="Nam Joong" last="Kim">Nam Joong Kim</name><name sortKey="Kwon, Nak Jung" sort="Kwon, Nak Jung" uniqKey="Kwon N" first="Nak Jung" last="Kwon">Nak Jung Kwon</name><name sortKey="Lee, Gir Won" sort="Lee, Gir Won" uniqKey="Lee G" first="Gir Won" last="Lee">Gir Won Lee</name><name sortKey="Oh, Myoung Don" sort="Oh, Myoung Don" uniqKey="Oh M" first="Myoung Don" last="Oh">Myoung Don Oh</name><name sortKey="Seo, Jeong Sun" sort="Seo, Jeong Sun" uniqKey="Seo J" first="Jeong Sun" last="Seo">Jeong Sun Seo</name><name sortKey="Seong, Moon Woo" sort="Seong, Moon Woo" uniqKey="Seong M" first="Moon Woo" last="Seong">Moon Woo Seong</name><name sortKey="Yun, Jiyoung" sort="Yun, Jiyoung" uniqKey="Yun J" first="Jiyoung" last="Yun">Jiyoung Yun</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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